Inclusion of catalase in semen extender modulates the post thaw semen quality and oxidative stress profiles in mithun spermatozoa

Present study was designed to assess the effect of catalase on post-thaw semen quality parameters (SQPs), sperm kinematics, antioxidant and oxidative stress profiles cholesterol efflux in mithun (Bos frontalis). A total 25 ejaculates were selected based biophysical each sample split into four equal aliquots diluted (to get final concentration 60 million spermatozoa per mL) with TCG extender containing either 0 U/mL, 50 100 150 U/mL Group I, II, III IV, respectively. Frozen-thawed samples analysed for motility (progressive forward bovine cervical mucus [BCMPT]), kinetic velocity by computer-assisted analyser (CASA), viability, morphological nuclear abnormalities, acrosomal integrity, plasma membrane integrities, intra-cellular enzymatic leakage seminal biochemical (sperm markers) profiles. Study revealed an enhancement mucus), content reduction intracellular enzymes III. Moreover, intactness acrosome membranes protected significantly addition significant improvement U/ml catalase. Correlation analysis that parameters, SQPs had positive correlation other whereas these negatively correlated lipid peroxidation treated sperm. The results clearly indicated inclusion holds a clear advantage over control or cryopreservation semen. It can be concluded from present supplementation effectively utilized reduce improve mithun.